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primary antibodies against lyve 1  (R&D Systems)


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    R&D Systems primary antibodies against lyve 1
    Primary Antibodies Against Lyve 1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 93 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+lyve+1+antibody/Rat+LYVE-1+Antibody/pm41926284-218-63-69
    Average 93 stars, based on 93 article reviews
    primary antibodies against lyve 1 - by Bioz Stars, 2026-10
    93/100 stars

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    Incubation:

    Article Title: Transcranial photobiomodulation improves insulin therapy in diabetic microglial reactivity and the brain drainage system.
    Article Snippet: .. The meninges were incubated in the blocking solution (a mixture of 0.2% Triton-X-100 and 10% normal goat serum in PBS) for 1 h, followed by incubation with Alexa Fluor 488-conjugated anti-Lyve-1 antibody (1:500; FAB2125G, R&D Systems, Minneapolis, Minnesota, USA), rabbit anti-Prox-1 antibody (1:500; ab101851, Abcam, Cambridge, United Kingdom) overnight at room temperature. .. Then, the meninges were incubated with Alexa Fluor 555 goat anti-rabbit IgG (H+ L) (1:500, A21429, Invitrogen, Molecular Probes, Eugene, Oregon, USA).

    Article Title: Therapeutic potential of formononetin in cirrhotic portal hypertension: modulating hepatic fibrosis, macrophage polarization, and lymphangiogenesis
    Article Snippet: For IF analysis, rehydrated paraffin-embedded live sections or fixed cells were treated with 0.3% Trition X-100 (#T8787, Sigma-Aldrich, USA) for 15 min and then blocked in 10% goat serum (#G1208, Servicebio, China) for 1 h at 37°C. .. The sections were then incubated overnight at 4°C with anti-CD68 antibody (1:50, #ab955, Abcam, UK) plus anti-iNOS antibody (1:50, #ab283655, Abcam, UK) or anti-CD163 antibody (1:200, #ab182422, Abcam, UK), anti-α-SMA antibody (1:100, #ab7817, Abcam, UK) plus anti-LYVE-1 antibody (1:100, #AF7939, R&D Systems, USA), or anti-iNOS antibody (1:100, #CY5993, Abways, China) plus anti-CD206 antibody (1:100, #60143-1-Ig, Proteintech, USA). .. Samples were then washed, and secondary fluorescent antibodies (1:1000, #A-21235, #A-11008, #A-21448, #A-21245, #A-11001, Invitrogen, USA) were prepared for incubation at room temperature in dark for 1 h. After washing, antifade mounting medium with DAPI (#G1407 Servicebio, China) was added to the samples for nuclei staining.

    Article Title: Photostimulation of lymphatic clearance of β-amyloid from mouse brain: a new strategy for the therapy of Alzheimer’s disease
    Article Snippet: .. To visualize the lymphatic system in meninges, the whole-mount meninges were incubated in the blocking solution (a mixture of 0.2% Triton-X-100 and 10% normal goat serum in PBS) for 4 h, followed by incubation with Alexa Fluor 488-conjugated anti-Lyve-1 antibody (1:500; FAB2125G, R&D Systems, USA) and rabbit anti-Prox-1 antibody (1:500; ab101851, Abcam, United Kingdom) overnight at room temperature. .. After washing, the meninges were incubated with goat anti-rabbit IgG (H + L) Alexa Fluor 561 (Invitrogen, USA).

    Article Title: Photostimulation of brain lymphatics in male newborn and adult rodents for therapy of intraventricular hemorrhage.
    Article Snippet: For analysis of dcLNs, the lymph nodeswere removed and fixed in PBS with 4% PFA overnight at 4 °C, and then fixed in 2% agarose, followed by sliced into 60 μm-thick sections using a vibratome (Leica VT1000,Germany). .. Thewholemounts ofmeninges and the sections of dcLNs were firstly washed 3 times (5min for each) with wash solution (0.2% Triton-X-100 in PBS), secondly incubated in the blocking solution (a mixture of 2% Triton-X-100 and 5% normal goat serum in PBS) for 1 h, followed by incubation with rat Alexa Fluor 488-conjugated anti-LYVE-1 antibody (1:500; Cat. No. FAB2125G, R&D Systems, Minneapolis, Minnesota, USA), rabbit anti-PROX-1 antibody (1:500; Cat. No. ab 101851, Abcam, Cambridge, United Kingdom), rat Alexa Fluor 647-conjugated anti-CD-31 antibody (1:500; Cat. No.102416, BioLegend, San Diego, USA) and rabbit anti-LYVE-1 antibody (1:500; Cat. No. ab 218535, Abcam, Cambridge, United Kingdom) for rat dcLNs overnight at 4 °C in PBS containing0.2%Triton-X-100 and0.5%normal goat serum.Next, themeningeswere incubated at room temperature for 1 h and then washed 3 times, followed by incubation with goat anti-rabbit IgG (H + L) Alexa Fluor 555 (1:500, Cat. No. A21429, Invitrogen, Molecular Probes, Eugene, Oregon, USA), and goat anti-rabbit IgG (H + L) Alexa Fluor 488 (1:500, Cat. No. A11008, Invitrogen, Molecular Probes, Eugene, Oregon, USA) to visualize LVs in rat dcLNs. .. The sections of whole meninges from mice as well as approximately 10 slices of dcLN per adult animal and 5 slices for PD4 newborn rats were imaged using a confocal microscope (LSM 710, Zeiss, Jena, Germany) with a ×20 objective (0.8 NA) or a ×60 oil immersion objective (1.46 NA).

    Article Title: Transcranial photobiomodulation improves insulin therapy in diabetic microglial reactivity and the brain drainage system
    Article Snippet: .. The meninges were incubated in the blocking solution (a mixture of 0.2% Triton-X-100 and 10% normal goat serum in PBS) for 1 h, followed by incubation with Alexa Fluor 488-conjugated anti-Lyve-1 antibody (1:500; FAB2125G, R&D Systems, Minneapolis, Minnesota, USA), rabbit anti-Prox-1 antibody (1:500; ab101851, Abcam, Cambridge, United Kingdom) overnight at room temperature. .. Then, the meninges were incubated with Alexa Fluor 555 goat anti-rabbit IgG (H + L) (1:500, A21429, Invitrogen, Molecular Probes, Eugene, Oregon, USA).

    Article Title: Transcranial photobiomodulation improves insulin therapy in diabetic microglial reactivity and the brain drainage system.
    Article Snippet: Then, the meninges were incubated with Alexa Fluor 555 goat anti-rabbit IgG (H+ L) (1:500, A21429, Invitrogen, Molecular Probes, Eugene, Oregon, USA). .. According to the above steps, the slices of dCLNs of mice were also incubated by Alexa Fluor 488- conjugated anti-Lyve-1 antibody (1:500; FAB2125G, R&D Systems, Minneapolis, Minnesota, USA) and anti-IFN-γ (1:500, 16731181, Invitrogen, Molecular Probes, Eugene, Oregon, USA), followed by incubation with Alexa Fluor 647 goat anti-rabbit IgG (H+ L) (1:500, A21245, Invitrogen, Molecular Probes, Eugene, Oregon, USA). .. And 100 μm brain slices were incubated by antiMki67 (1:500 dilution; K009725P, Solarbio®, Beijing, China), followed by incubation with Alexa Fluor 555 goat anti-rabbit IgG (H+ L) (1:500 dilution; A21429, Invitrogen, Carlsbad, CA, USA).

    Article Title: Photostimulation of lymphatic clearance of β-amyloid from mouse brain: a new strategy for the therapy of Alzheimer's disease.
    Article Snippet: .. Frontiers of Optoelectronics (2023) 16:45 1 3 45 Page 4 of 12 To visualize the lymphatic system in meninges, the whole-mount meninges were incubated in the blocking solution (a mixture of 0.2% Triton-X-100 and 10% normal goat serum in PBS) for 4 h, followed by incubation with Alexa Fluor 488-conjugated anti-Lyve-1 antibody (1:500; FAB2125G, R&D Systems, USA) and rabbit anti-Prox-1 antibody (1:500; ab101851, Abcam, United Kingdom) overnight at room temperature. .. After washing, the meninges were incubated with goat anti-rabbit IgG (H + L) Alexa Fluor 561 (Invitrogen, USA).

    Article Title: Transcranial photobiomodulation improves insulin therapy in diabetic microglial reactivity and the brain drainage system
    Article Snippet: Then, the meninges were incubated with Alexa Fluor 555 goat anti-rabbit IgG (H + L) (1:500, A21429, Invitrogen, Molecular Probes, Eugene, Oregon, USA). .. According to the above steps, the slices of dCLNs of mice were also incubated by Alexa Fluor 488-conjugated anti-Lyve-1 antibody (1:500; FAB2125G, R&D Systems, Minneapolis, Minnesota, USA) and anti-IFN-γ (1:500, 16731181, Invitrogen, Molecular Probes, Eugene, Oregon, USA), followed by incubation with Alexa Fluor 647 goat anti-rabbit IgG (H + L) (1:500, A21245, Invitrogen, Molecular Probes, Eugene, Oregon, USA). .. And 100 μm brain slices were incubated by anti-Mki67 (1:500 dilution; K009725P, Solarbio ® , Beijing, China), followed by incubation with Alexa Fluor 555 goat anti-rabbit IgG (H + L) (1:500 dilution; A21429, Invitrogen, Carlsbad, CA, USA).

    Blocking Assay:

    Article Title: Transcranial photobiomodulation improves insulin therapy in diabetic microglial reactivity and the brain drainage system.
    Article Snippet: .. The meninges were incubated in the blocking solution (a mixture of 0.2% Triton-X-100 and 10% normal goat serum in PBS) for 1 h, followed by incubation with Alexa Fluor 488-conjugated anti-Lyve-1 antibody (1:500; FAB2125G, R&D Systems, Minneapolis, Minnesota, USA), rabbit anti-Prox-1 antibody (1:500; ab101851, Abcam, Cambridge, United Kingdom) overnight at room temperature. .. Then, the meninges were incubated with Alexa Fluor 555 goat anti-rabbit IgG (H+ L) (1:500, A21429, Invitrogen, Molecular Probes, Eugene, Oregon, USA).

    Article Title: Photostimulation of lymphatic clearance of β-amyloid from mouse brain: a new strategy for the therapy of Alzheimer’s disease
    Article Snippet: .. To visualize the lymphatic system in meninges, the whole-mount meninges were incubated in the blocking solution (a mixture of 0.2% Triton-X-100 and 10% normal goat serum in PBS) for 4 h, followed by incubation with Alexa Fluor 488-conjugated anti-Lyve-1 antibody (1:500; FAB2125G, R&D Systems, USA) and rabbit anti-Prox-1 antibody (1:500; ab101851, Abcam, United Kingdom) overnight at room temperature. .. After washing, the meninges were incubated with goat anti-rabbit IgG (H + L) Alexa Fluor 561 (Invitrogen, USA).

    Article Title: Photostimulation of brain lymphatics in male newborn and adult rodents for therapy of intraventricular hemorrhage.
    Article Snippet: For analysis of dcLNs, the lymph nodeswere removed and fixed in PBS with 4% PFA overnight at 4 °C, and then fixed in 2% agarose, followed by sliced into 60 μm-thick sections using a vibratome (Leica VT1000,Germany). .. Thewholemounts ofmeninges and the sections of dcLNs were firstly washed 3 times (5min for each) with wash solution (0.2% Triton-X-100 in PBS), secondly incubated in the blocking solution (a mixture of 2% Triton-X-100 and 5% normal goat serum in PBS) for 1 h, followed by incubation with rat Alexa Fluor 488-conjugated anti-LYVE-1 antibody (1:500; Cat. No. FAB2125G, R&D Systems, Minneapolis, Minnesota, USA), rabbit anti-PROX-1 antibody (1:500; Cat. No. ab 101851, Abcam, Cambridge, United Kingdom), rat Alexa Fluor 647-conjugated anti-CD-31 antibody (1:500; Cat. No.102416, BioLegend, San Diego, USA) and rabbit anti-LYVE-1 antibody (1:500; Cat. No. ab 218535, Abcam, Cambridge, United Kingdom) for rat dcLNs overnight at 4 °C in PBS containing0.2%Triton-X-100 and0.5%normal goat serum.Next, themeningeswere incubated at room temperature for 1 h and then washed 3 times, followed by incubation with goat anti-rabbit IgG (H + L) Alexa Fluor 555 (1:500, Cat. No. A21429, Invitrogen, Molecular Probes, Eugene, Oregon, USA), and goat anti-rabbit IgG (H + L) Alexa Fluor 488 (1:500, Cat. No. A11008, Invitrogen, Molecular Probes, Eugene, Oregon, USA) to visualize LVs in rat dcLNs. .. The sections of whole meninges from mice as well as approximately 10 slices of dcLN per adult animal and 5 slices for PD4 newborn rats were imaged using a confocal microscope (LSM 710, Zeiss, Jena, Germany) with a ×20 objective (0.8 NA) or a ×60 oil immersion objective (1.46 NA).

    Article Title: Transcranial photobiomodulation improves insulin therapy in diabetic microglial reactivity and the brain drainage system
    Article Snippet: .. The meninges were incubated in the blocking solution (a mixture of 0.2% Triton-X-100 and 10% normal goat serum in PBS) for 1 h, followed by incubation with Alexa Fluor 488-conjugated anti-Lyve-1 antibody (1:500; FAB2125G, R&D Systems, Minneapolis, Minnesota, USA), rabbit anti-Prox-1 antibody (1:500; ab101851, Abcam, Cambridge, United Kingdom) overnight at room temperature. .. Then, the meninges were incubated with Alexa Fluor 555 goat anti-rabbit IgG (H + L) (1:500, A21429, Invitrogen, Molecular Probes, Eugene, Oregon, USA).

    Article Title: Photostimulation of lymphatic clearance of β-amyloid from mouse brain: a new strategy for the therapy of Alzheimer's disease.
    Article Snippet: .. Frontiers of Optoelectronics (2023) 16:45 1 3 45 Page 4 of 12 To visualize the lymphatic system in meninges, the whole-mount meninges were incubated in the blocking solution (a mixture of 0.2% Triton-X-100 and 10% normal goat serum in PBS) for 4 h, followed by incubation with Alexa Fluor 488-conjugated anti-Lyve-1 antibody (1:500; FAB2125G, R&D Systems, USA) and rabbit anti-Prox-1 antibody (1:500; ab101851, Abcam, United Kingdom) overnight at room temperature. .. After washing, the meninges were incubated with goat anti-rabbit IgG (H + L) Alexa Fluor 561 (Invitrogen, USA).



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